A Study On The Resonance Rayleigh Scattering Spectra Of The Interactions Of Gold Nanoparticle With Some Alkaloids, Protein Of Basic Dye And Their Analytical Applications

Gold Nanoparticle has various especial physical and chemical properties, the study on the interactions of gold nanoparticle with organic or biologic molecule and their spectral characteristics has important significance in theory and wide foreground in the applications. Recently, as the new highly sensitivity and simple analytical technique, resonance Rayleigh scattering (RRS) and resonance nonlinear scattering (RNLS) methods bring to more attention and interesting. In this paper, we investigated RRS and RNLS spectra of the interaction of Gold nanoparticle with some Alkaloids, Protein or Basic Dye. The spectral characteristics, the optimum conditions of the reactions, the affecting factors, the properties of analytical chemistry and their analytical application have been studied. 1. Gold nanoparticle-some alkaloids systems 1.1. Gold nanoparticle-berberine hydrochloride systemIn pH 4.0 citrate natrium-hydrochloride buffer solution, gold nanoparticle and berberine hydrochloride react with each other to form the aggregation, which make the diameters of the particles increscent, the diameter of the aggregation is incresed from 12nm to 35nm, therefore, the RRS intensity can be greatly enhanced. The peaks of this system locate at 306nm, 365nm and 495nm. In this paper, we choose 495nm as the determined wavelength. The optimum conditions for the reactions, the characteristics of RRS spectra, the sensitivity and selectivity have been investigated. The RRS intensity is directly proportional to the concentration of berberine hydrochloride in the range of 0.08~0.24ug/mL. The method has high sensitivity, and the detection limit for berberine hydrochloride is 0.4ng/mL. Under the reaction condition, most metal, non-metal ions and glucide do not interfere, so that the method has a good selectivity. Therefore, a method for the determination of berberine hydrochloride by RRS was developed. It was applied to determine the berberine hydrochloride in the real samples with satisfactory results.1.2 Gold nanoparticle-quinine sulphate systemIn pH 4.4 citrate natrium-hydrochloride buffer solution, gold nanoparticle and quinine sulphate react with each other to form the aggregation, which make the diameters of the particles increscent, the diameter of the aggregation is incresed from 12nm to 30nm, therefore, the RRS intensity can be greatly enhanced. The peaks of this system locate at 306nm, 365nm and 495nm. In this paper, we choose 495nm as the determined wavelength. The optimum conditions for the reactions, the characteristics of RRS spectra, the sensitivity and selectivity have been investigated. The RRS intensity is directly proportional to the concentration of quinine sulphate in the range of 14.4~50ng/mL. The method has high sensitivity, and the detection limit for quinine sulphate is 0.23ng/mL. Under the reaction condition, most metal, non-metal ions and glucide do not interfere, so that the method has a good selectivity. Therefore, a method for the determination of quinine sulphate by RRS was developed. It was applied to determine the quinine sulphate in the real samples with satisfactory results.2. Gold nanoparticle-protein systemIn pH 3.0 citrate natrium-hydrochloride buffer solution, protein and gold nanoparticle aggregate with each other depending on the static gravitation and hydrophobic force to form the aggregation, the RRS intensity can be greatly enhanced. The characteristics of RRS spectra, the optimum conditions, the sensitivity and selectivity have been investigated. The RRS intensity is directly proportional to the concentration of proteins. The method has high sensitivity, and the detection limits for protein are 0.38ng/mL for the human serum albumin system, 0.45nm/mL for the bovine serum albumin system and 0.56ng/mL for the ovalbumin system. Under the reaction condition, besides aminophenol, most metal, non-metal ions and glucide do not interfere, so that the method has a good selectivity. Therefore, the method for the determination of protein by RRS was developed. It was applied to determine the...