A Study On Enrichment Of Linoleic Acids And Preparing Conjugated Linoleic Acids In Safflower Oil

Experiment1: Research on enrichment of linoleic acids in safflower oil by urea adduct method showed that the amount of urea affected deeply on enrichment of linoleic acid. The fatty acid iodine value were 62.7% and the recovery ratio were 42% in filtrate when the ratio between urea(w/w) and fatty acid was about 3:1. Ethanol as a solvent affected the concentration of reactants in urea inclusion. Iodine value and recovery ratio of the fatty acid in filtrate was the highest when the ratio between ethanol(95%) and fatty acid(V/W) was 18:1. Crystallization temperature influenced directly the kinds, shape and size of urea adducts. The reaction was incomplete with over-increase of temperature and the recovery ratio was over-low with decrease of temperature. Crystallization time had no effect on reaction. The optimum reaction conditions were urea : fatty acid(W/W)=3:1, ethanol (95%) : fatty acid(V/W) = 18:1 and crystallization reacted 18 hours under 5℃.The amount of linoleic acids were increased from 71% to 94% under the optimum reaction condition, which was used as a base for industrial designs and provided material for the following reaction.Experiment2:The enriched fatty acid by urea adduct method in safflower oil reacted by alkali isomerization. Reaction conditions such as alkali, solvent, time and temperature were determined by orthogonal design.The results showed that the alkali influenced significantly the productivity of conjugated linoleic acid(CLA),and the reaction occoured entirely when fatty acid : KOH(W/W)=2:1 or 3:1. Solvent as a reaction medium had no significant effect but it increased the reclaim cost of solvent. With reaction time increasing, productivity of CLA increased , but occure ratio of side reaction increased also, so the amount of CLA don't increase when the reaction time reached a specific level. Temperature could influence significantly the rate of reaction. High temperature enhanced the reaction rate , but aggravated the colour of product, which made the separation and purification of the product be more difficult. The data of experiment were dealt by using the method of GLMN mulifactor root deviation analysis and doing DUNCUN of multiple comparison. The result showed the optimum reaction conditions were fatty acid:KOH(W/W)-2:l, solvent.fatty acid(V/W)=4:l and respond 4 hours under 190℃. The CLA content was 72.41% in mixed fat acids .Experiments:The experiment studied the effect of different reaction conditions such as the ratio of gelatin to Arabic gum as compound well material, the PH value of polymer and the entering wind temperature of spying on microencapsulating reaction. The results showed that the optimum reaction conditions were following: the ratio of gelatin and Arabic gum ratio was 1.3:1, the PH value was 4.0 and the temperature was 200℃. High quality microencapsulated conjugated linoleic acid with higher microencapsulation ration of 92.41% and lower surface oil of 3.42% were made under the optimum reaction conditions . The anti-oxidative ability of the product was better than original oil.