The Application Of Baker's Yeast In Selective Bioreduction

Bioreduction of some compounds containing C=O double bond, C=C double bond and C=N double bond with Baker's Yeast was developed. Those compounds containing C=N double bond involve N-phenyl-benzylimine, prepared from benzaldehyde and aniline, N-phenyl-2-hydroxy-benzylimine and N, N'-disalicylidene- ethylenediamine, synthesized by the reaction of salicylaldehyde with aniline and ethylenediamine respectively, as well as N, N'-bis-(1-thiophen -2-yl-ethylidene)-ethane-1,2-diamine, derived from ethylenediamine and 2-acetyl-thiophen, which was synthesized with thiophen as the starting material. Baker's Yeast-catalyzed reduction was studied by the use of Dry Baker's Yeast Process (DBYP), Cultured Baker's Yeast Process (CBYP) and Immobilized Baker's Yeast Process (IBYP). The examination of those three processes and the comparison between them are also studied in this process. Among those processes, DBYP was the simplest and the most convenient, in the same way, the character of CSPBY, which employed the freshly cultured Baker's Yeast, lied in the high activity and the convenience of the separation of products. IBYP, however, had the demand for the immobilization of cells of Baker's Yeast in the microcapsule of calcium alginate, which allowed the reaction in organic solvent, but lowered the activity of Baker's Yeast.