Study On Compositions Of Chinese Herbs Against Endometritis Of Cows

According to "Veterinary Pharmacopoeia of PRC. " and a great deal of references on Chinese herbs ,twelve kinds of Chinese herbs usually used to treat endometritis of cows were chosen ,which are Rhizoma Coptidis, Cortex Phellodendri, Rhizoma Rhei, Radix Scutellariae, Radix Sophorae Flavesentis, Fructus Forsythiae, Radix Pulsatillae, Herba Leonuri, Flos Carthami, Fructu Cnidii, Folium Artemisiae Argyi and Radix Glycyrrhizae. Their extracting processes were optimized by orthogonal test and activities of extracts determined by antibiotic test in vitro.The results showed that most of the extracts have inhibition to the main pathogens Staphylococcus aureus and Escherichia coli causing endometritis of cows, and different extracts have different inhibition to a kind of pathogen.Under the direction of Chinese Medical Theory, fifteen compositions were designed on the basis of the twelve kinds of herbs and their extracts that the above mentioned. Their activity against Staphylococcus aureus and Escherichia coli were determined by antibiotic test in vitro and compared with three traditional compositions. Seven compositions with better activity were further sorted out, whose components and proportions are as follows:Composition 1: Cortex Phellodendri, Rhizoma Coptidis and Rhizoma Rhei in the proportion of 1:1:2 2: Cortex Phellodendri, Rhizoma Coptidis and Rhizoma Rhei in the proportion of 1:1:1 3: Radix Sophorae Flavesentis and Radix Pulsatillae in the proportion of 1:1 4: Radix Sophorae Flavesentis and Radix Pulsatillae in the proportion of 1:2 9: Cortex Phellodendri, Rhizoma Rhei, Radix Sophorae Flavesentis, Folium ArtemisiaeArgyi and Fructu Cnidii in the proportion of 3:3:3:1:21': Cortex Phellodendri, Rhizoma Coptidis, Radix Scutellariae, Fructus Forsythiae, Radix Sophorae Flavesentis and Radix Glycyrrhizae in the proportion of 20:5:13:13:10:53': Cortex Phellodendri, Rhizoma Coptidis, Radix Scutellariae, Radix Sophorae Flavesentis, Herba Leonuri and Flos Carthami in the proportion of 15:10:10:8:10:7...