Study On The Isolation And Extraction Of Active Peptides From Rana Chensinensis Skin

Chinese Forest Frog (Rana chensinensis) is a kind of important amphibian in China and also one of the most important medical animals of Hei Longjiang Province. At present, there are plenty of reports on the contents and functions of the Oviductus Ranae about the study of Chinese Forest Frog, but there have done little of work on the study and exploitation of active substance distributed in the skin of Chinese Forest Frog. There are many bioactive substances in the skin of frogs, which have different special molecule structures and complicated biological functions. These bioactive substances have important value in clinical application, and great potential for the exploitation and utilization of these resources. As much, there are lots of works will be done. It will be a firm foundation of more effective exploitation and utilization of Chinese Forest Frog resource, if some important value bioactive substances can be isolated and extracted from the skin of Chinese Forest Frog, and richen the "North medicine" resources.The experiment chose the skin of Chinese Forest Frog bought from the "Maoer mountain" of Hei Longjiang province as experimental material. The optimal extracted condition of active peptides and technical route was confirmed through conditional experiment of extracted system, extracted temperature, extracted hour. The data of SDS-PAGE electrophoresis, which analyzed small molecule peptides was confirmed through probing, and established the experiment project of isolation and purification of active peptides in frog skin by PAGE electrophoresis.Antibiotic test was chose to examine the functions of active peptides in the skin of Chinese Forest Frog. Bacterium: E. coli, Pseudomonas, Staphylococcus aureus, Actinomyces. The result suggests that the small molecule bioactive peptide in the skin of Forest Frog has ability against gram-positive bacterium and gram-negative bacterium. This indicates active peptides of Forest Frog skin have great potential for the exploitation and utilization.