Study On Biological Effect Of Component Of Cellulase By Ion Implantation

The cellulose is the biggest reborn functional source material on the earth, its degradation and conversion by microorganism are very important for carbon cycle in nature, moreover the valid exploitation of this process may develop the enormous function on supplying energy feedstuff and food . Base on this idea , International community microorganism worker work hard for studying microorganism which produce cellulaseoThis thesis proceeded as follows of research .The filtrate of Hy2 and Hya-3311 precipitate by (NH4)2SO4 ,Sephadex G-25 desalination ,Sephadex G-75 column chromatography and ion exchange chromatography on DEAE-Sephadex A-50.The results showed that Cellulase components changed by ion implantation ,especially B - glucosidase components increased and other components as well. Moreover the quality of pure enzyme also changed : the most suitable temperature and the most suitable pH are increased by ion implantation; but temperature stability and the stability of pH are decreased by ion implantation. It is discovered change which capacity of cellulase combine DEAE-Sephadex A-50 o And eluted with NaCl , the Hy2-3311 needs concentration is 0.30-0.50 mol/L,but Hy2 is only 0.5 mol/L. Mensurate synergetic effect , it is found that both the ability of decomposition fusible cellulose and infusible cellulose is increased by ion implantation. In dynamics study , It was discovered that the dynamics model of cellulase changed by ion implantation , This research result confirmed that the cellulase surface structure was changed ,and it was this change induce that the efficiency increase of cellulase decomposing. It was suggested that the genie change of cellulase caused by ion implantation.