| Chloramphenicol (CAP) is a broad-spectrum antibiotic, Because of its toxic properties, it has been banned from producing animal foods in the European community and the United States at levels greater than 0. lug/kg. Several analytical methods for the ^determination of CAP in different biological materials, based on gas chromatography, gas chromatography mass spectrometry, liquid chromatography have been published. But GC and HPLC can not satisfied with analytical requirement because of low sensitivity. GC-MS method has demonstrated excellent sensitivity ,but The sample treatment is time-consuming, require derivatization.We analyzed CAP residues in producing animal foods using Liquid Chromatography/Electro-spray Ion Trap . The sample are extracted with liquid/liquid extraction or solid-phase extraction ,The sample treatment is simple. LC conditions and MS parameters are optimized, the limit ofquantification for CAP by LC-MS is lower than 0. lug/kg, RSD isLiquid chromatography/Quadrupole and Liquid chromatography/Ion Trap methods for determination of CAP residues are compared. The Limit of Detection for Liquid Chromatography/Ion Trap detection is 0.02ug/kg while the corresponding value for Liquid chromatography/Quadrupole detection is 0.2ug/kg.And MRM techniques for Ion Trap detection can filter most of matrix in food, Qualitative and quantitative analysis using MS/MS, The result are less controversial .and it is obvious that the sample matrix interferes with the sensitivity using Quadrupole detection.Atmospheric pressure photoionization is a new ionization techniques for LC/MS, and Liquid Chromatography-Atmospheric Pressure Photoionization- Quadrupole Mass Spectrometry method is developed for the determination of CAP antibiotics in food, an important advantage of using APPI for CAP is that sample matrix did not significantly affect ion intensity of CAP, The Limit of detection is 0.10-0.27ug/kg,RSD is 4.3%~6.7%.
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