Electrochemistry And Electrochemical SPR Biosensors Based On Gold Electrode

Biosensor is a new subject based on biology,chemistry,physics,medicine and electronics. The manufacture of biosensor is a focus project in many research field recently. Based on the formal research, this thesis studied as follows:The direct electron transfer of ferritin covalently on modified gold electrode is investigated. Cyclic voltammetry was the principal method used to investigate the reactivity of the adsorbed layer. Moreover, the process of modified electrode was characterized by SPR. On this modified electrode, ferritin exhibits resonably well-defined current-potential curves. In addition, the mechanism of electrochemically regulated iron uptake and release for ferritin was obtained.Two important enzymes were imoblized using the electrostatic interaction of molecular and the biological specific interaction. The surface plasmon resonance(SPR) technique,cyclic voltammetry method and electrochemistry impedance spectrum (EIS) were used to characterize this ultrathin film. It shows that this kind of film was uniform,steady and electro-active. It may be used to construct the biosensors and biofuel cells.The thin constructed in chapter 3 were used to construct the glucose biosensor. In-situ electrochemistry surface plasmon resonance (ESPR) was used to detect the glucose concentration. It shows it is possible to detect the bioelectro-enzymatic reaction using the ESPR technique. SPR technique may be complemented to the electrochemistry measurement. Moreover, quantitative information of the redox enzyme reaction through the two parameters may be obtained. This approach can also be extended by using other oxidoreductases and applied to the measurement of different substrates.