The Study On Liquid Fermentation And Application Of Actinomucor Elegans Protease

Actinomucor elegans is one of the primary strains used in the fermentation of sufu .The fermentation of mucor protease in condition of liquid submerged culture of AS3.2778 and the characteristics of the protease were studied in this research.The Application of mucor protease in the novel ways of sufu fermentation were also studied in this paper.The results of the experiments showed that Actinomucor elegans AS3.2778 can produce mucor protease well at 28 ℃, initial pH 8.5 on a rotary shaker with a speed of 220~250 r/min. The constitution of medium of protease produced by Acdnomucor elegans were optimized through Uniformity Design .Its growth accorded with the Growth Model of Rogistic, and the rule of mucor protease production fits the Luedeking-Piret's Equation.The study of mucor protease characteristics showed that mucor protease belonged to neutral protease, the ideal conditions for the protease activity were 40~55℃, pH 7~8.The distribution of the protease MWs were above 30 kD,and were made up of high and medium parts.In order to improve the traditional sufu fermentation and apply the up-to-date bio-technique to the manufacturing process of sufu, we made research on producing paste-pattern sufu with enzymes.The optimum technical conditions for hydrolyzation were as follows : the concentration of substrate was 14% , the concentration of protease was 374 U/g, the temperature of hydrolyzation was 40 ℃, the pH was 8.5. The hydrolyzation were followed by the experiments of mixed culture,adjusting texure and taste .The quality of final product could meet the quality index of commercial red sufu.It was considered that our reaserch might be useful for producing sufu with enzymes.