The Study On Azo Dye Decolorization By Genetical Engineering Microorganism

Some technologies to treat azo dye wastewater are introduced in this paper, especially the characters and application of genetic engineering technology and bioaugmentation. Four aspects are included in the paper, the optimization condition of JM109 (pGEX-AZR) fermantation, the the study on decolinzation of JM109 (pGEX-AZR), the study on decolorization of JM109, the bioaugmentation of two bacteria.The optimal fermentation condition of JM109 (pGEX-AZR): Na2HPO4 (4.26g/L) , KH2PO4(2.65g/L), MnSO4-7H2O(0.002g/L), FeSO4-7H2O(0.01g/L), MgSO4-7H2O(0.2g/L), CaCl2(0.02g/L), NH4Cl(0.5g/L), Amp.(50mg/L), glucose (5g/L), NH4Cl (3g/L), pH 7.5, temperature35℃, inoculation quality10mL/LUnder anaerobic condition, JM109 (pGEX-AZR) can decolorize more efficiently than aerobic. It can decolorize numerous azo dyes, such as acid red red B, acid orange II, acid black 10B, acid red G, acid red GR. The decolorization concentration scale is 50~5000mg/L. The optimal decolorization condition is pH6~8,25-40 ℃, salinity 1-5%. The decolorization accords with the first class kinetics equation.Under anaerobic condition, E. coli JM109 can decolorize more efficiently than aerobic. It can decolorize numerous azo dyes, such as acid red B, acid orange II, acid black 10B, acid red G, acid red GR. The decolorization rate is 80% at the concentration of 5000mg/L. The optimal decolorization condition is pH6~8, 25-40 ℃, salinity 1-5%.Compared with the activated sludge, the two systems bioaugmented by JM109 (pGEX-AZR) and E.coli JM109 treat azo dye wastewater more efficiently, especially JM109 (pGEX-AZR). The optimal glucose concentration of influx is 1000mg/L. JM109 (pGEX-AZR) can bioaugment the treat of acid red B, active red K-2BP and acid fuchsin 6B.