Study On Nerve Toxicity And Oxidation Damage To Antioxidant Defenses Of Mice Induced By Pentachloronitrobenzene (PCNB)

This paper selected disinfectant pentachloronitrobenzene (PCNB) as research object,studied its nerve toxicity and the damage on oxidize defend system on mice withacetylcholinesterase(AChE), catalase(CAT), acetylcholine(ACh) and lipid peroxidation(LPO)at the molecular level of toxicity. Moreover, this paper tried to probe into the effectmechanisms of its nerve toxicity and oxidation damage, which provided the basis for the fullyestimate of ecological toxicology on mammals. The toxicant dose was 200mg/kg, 400mg/kgand 600mg/kg, and fed mice 4,8,12 and 16 days respectively each dose. The results showedthat with the concentration increasing, the activities of CAT in brain descended, the level ofACh in liver had a tendency to increase, and showed an evident dependence on dose and time,indicated that PCNB had nerve toxicity. Following the dose increasing, the activities of CATof brain and serum increased in groups of 4 and 8 days, while decreased in groups of 12 and16 days; in liver, the activities of CAT were descending evidently at the beginning of toxicosis;in kidney, the activities of CAT were increasing all through the toxicosis time with theconcentration increasing and time extending. To the level of LPO, the level of MDA of brainhad a tendency to decrease with PCNB concentration increasing all the time, showed nooxidation occurred in it; while it is just contrary in liver and kidney which indicated thatoxidation was serious in this two organs; the level of MDA of spleen increased distinctly onlyat the end of toxicosis time and had no great change in serum. Through experiment results wecould take a conclusion that PCNB damaged liver and kidney of mice badly and the brain andspleen were also injured at a certain extent. Through observed by eyes and viscera coefficientchanging we concluded that the liver tumescented, and the spleen atrophied.