| Neoglycoprotein was prepared from silver carp myofibrillar protein by glycosylation reaction with lactose and glucose respectively, and its functional properties were determined. The primary results are as follows:1. The lyophilized protein mixed lactose(weight rate of lactose and myofibrillar '. 1:1, 2:1, 4:1, 6:1) was incubated at 50℃(0 —48h) and 55℃(0~48h) and 65% relative humidity. Solubility of neoglycosylation in 0.1 and 0.5mol/L NaCl was determined, and conjugation site was researched by SDS-PAGE analysis. These results indicated that solubility of myofibrillar proteins in 0.1mol/L NaCl solution was improved by glycosylation reaction, and myosin heavy chain was selectively conjugated with lactose.2. The condition of glycosylation reaction was optimized by RSM, and the condition was confirmed, in which myofibrillar-lactose and myofibrillar-glucose with better functional properties was prepared. The results are as follows:(1) The condition improved solubility of myofibrillar-lactose is 50℃, reaction time 18.5h, weight rate of lactose and Mf 1.5; the condition improved emulsifying activity of myofibrillar-lactose is 50℃, reaction time 18.5h, weight rate of lactose and Mf 2.15; the condition improved emulsifying stability of myofibrillar-lactose is 50℃, reaction time 18.5h, weight rate of lactose and Mf 1.5; the condition improved thermal stability of myofibrillar-lactose is 55 ℃, reaction time 13h, weight rate of lactose and Mfl.5.(2) The condition improved solubility of myofibrillar-glucose is 45 ℃, reaction time 13h, weight rate of glucose and Mf 1.5; the condition improved emulsifying activity of myofibrillar-glucose is 55℃, reaction time 18.5h, weight rate of glucose and Mf 0.85; the condition improved emulsifying stability of myofibrillar-glucose is 55 ℃, reaction time 24h, weight rate of glucose and Mf 0.2; the condition improved thermal stability of myofibrillar-glucose is 45 ℃, reaction time 13h, weight rate of glucose and Mf 2.15.
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