Study On The Biological Characterization Of The Mutants Of Heavy Metal-Tolerant Penicillium Janthinellum GXCR1

The growth characteristics of Penicillium janthinellum GXCR1 and its mutants, MGXCR1, SM1 and SM2, were studied. It showed that the optimum growth temperature of SM2 conformed to the GXCR1 were 32 ℃, of the MGXCR1 and SMI was 28℃ and 37℃ respectively. The optimum growth pH value of the fungi was 5.0-7.0. The fungous ability of resisting and adsorbing different heavy metal was studied. It showed that the ability of resisting CuSO₄ , ZnSO₄ and Al₂(SO₄)₃ in turn was:GXCR1>MGXCR1>SM2>SM1, of resisting MnSO₄ in turn was: SM2>GXCR1>MGXCR1> SM1. The ability of adsorbing Cu²⁺ in turn was GXCR1>SM2>MGXCR1>SM1; of adsorbing Zn²⁺was: SM2 >MGXCR1> SM1>GXCR1; of adsorbing Al³⁺ was: GXCR1>SM1>MGXCR1>SM2; of adsorbing Mn²⁺ was: SM2 >MGXCR1 >GXCR1> SM1. The fungous ability of resisting different heavy metal and the ability of adsorption was not directly interrelated. The effect of time, pH, and heavy metal on the production of the extracellular protein and the pH of extracellular production was inconsistent to different fungous. Extracellularprotein consistency and content of different fungous were inconsistent too.