Synthesis And Characterization Of The Binuclear Complexes And Their Research For Cleaving DNA

In vivo, there occurs the synthesis and degration of the nucleic acids that concerning the formation and cleavage of phosphodiester bond. The cleavage modes of phosphodiester bond can be divided into oxidative and hydrolytic, only the later is connectable, can be religated by ligases. In recent years, a great progress has been made in chemical mimic for the hydrolytic cleavage of phosphodiester bond by using bioinorganic methods. Among the metal complexes been found with the ability to cleave nucleic acids in hydrolytic mode, there are mononuclear metal complexes, as well as dinulear complexes, and the metal ions being transitional and lanthanide metal ions, but the reaction speeds are still smaller for several grades than that of the natural enzymes. It should be a prosperous direction to mimic the hydrolytic cleavage of phosphodiester bond by taking the advantage of multinuclear metal complexes.Investigating the structure and activity of chemical nuclease, finding and synthesizing more effective chemical nuclease have attracted great attention. Such a situation is benefited form the cognition of the importance that the active centers of a number of natural enzymes in relation to nucleic acid usually have two or three cooperative metal ions, such as Zn(II), Mg(II), Ni(II) etc. Meanwhile, research about using rare-earth ions and their complexes as artificial restriction enzymes has gotten more and more recognition, as they can hydrolytically cleave the phosphate diester bond in nucleic acid, and their cleavage efficiencies are higher.In this dissertation, a series of ligands R-HXTA (R-HXTA=N,N'-(2-hydroxy -5-R-l,3-xylylene)bis(N-carboxymethylglycine)) were synthesized, where R is respectively -CH3, -Cl, -NO2 respectively, and we signed these ligands as L1, L2, L3. They can supply two same coordination environment arm for two metal ions. We have synthesized some new dinuclear metal complexes of these ligands and studied interaction and reaction mode between complexes and DNA. The thesis includes as the follows:...