Purification And Characterization Of Fibrinolytic Enzyme And Thrombin-Like Enzyme From Agkistrodon Acutus Venom

Nowadays, the diseases of blood coagulation system have been concerned for their mortality and morbidity. There are more than five million patients who were suffering for this kind of diseases every year in China. Many a pharmaceuticals are required to be researched and developed. Compared with congener products, venom, as a kind of biological preparation, has lots of advantages such as high efficiency, fewer by-effects, and secure usage, and would be extensive applied in clinic. However, the researches about the utilization of venom enzymes are most focused on a single enzyme. It would waste many biological resources. Therefore it is necessary that as many as possible enzymes should be isolated from the same venom preparation.Agkistrodon acutus venom was fractionated by chromatographies with Sephadex G-75 and DEAE respectively. The two purified enzymes were subjected to HPLC and SDS-PAGE. The result of experiments shows that the enzymes are both single peeks on C18 HPLC and single bands on SDS-PAGE. The molecular mass of fibrinolytic enzyme is 24.1kD, and that of thrombin-like Enzyme is 14.4kD.This article describes a simple method to isolate fibrinolytic enzyme and thrombin-like enzyme from the same venom preparation. The result shows that special activities of the two different enzymes purified were...