Studies Of Environmental Hormone Atrazine On The Toxicology Of Silkworm, Bombyx Mori

OBJECT AND METHODS: To find out the effect of environmental hormone atrazine (AT) on lepidopteron, selected Bombyx silkworm as the experimental animal for the first time. Feed the Bombyx larvae with artificial diet which added the AT, then survey the toxicity on its bionergy and fecundity. Cultured BmN cells in vitro, then survey the effect on the cells development and apoptosis by methods of MTT [3- (4,5) dimethylthiazol-2, 5-diphenyl-tetrazolium bromide] SCGE (single-cell gel electrophoresis) DAPI (4,6-diamidino-2-phenylindole) and FCM (flow cytometer).RESULTS. As feed the Bombyx larvae with diet that added 0.05~0.40mmol/kg AT, the growth speed, weight, developmental uniformity, bionergy and fecundity were negatively affected if AT exceeds 0.10mmol/kg. The LC50 value of AT on the setae dispersion rate of Haoyue ant silkworm larvae is about 0.38mmol/kg. As the concentration rise to 0.40mmol/kg, the silkworm even can't complete their generations. At 0.80mmol/kg, the larvae will die rapidly for the toxicity. AT can notably restrain the survival rate of BmN cells when over 0.0625mmol/L after 48 hours; the LC50 is 0.0700mmol/L. Treated with 0.05~0.40mmol/kg AT 24h after, then the nucleolus of BmN begins to apoptosis and shows concentration effect clearly. It can deduce the DNA breakage of the BmN cells deal with AT 0.5000mmol/L after 48 hours.CONCLUSION: AT shows pernicious effect of environmental hormone on lepidopteron of silkworm.