The Study On Molecule Recognition Effect Of Carbon Fiber Microelectrode And Calixarene Chemical Modified Electrode

The thesis is made up of two parts. In the first part, the paper elaborately introduces the importance of analysis and determination neurotransmitters and sums up the development on the field of microelectrode. The preparation and pretreatment of the Carbon fiber microelectrodes and the investigation production in the field of neurotransmitters were studied carefully. The interaction of serotonin was studied electrochemically by using voltammetry at the self-made carbon fiber microelectrodes in 0.05 mol·L~-1 phosphate buffer (pH6.0). The main work and results are summarized as following: (1) Self-made carbon fiber microelectrodes were pretreated by potentiostatic activation. The pretreated microelectrodes exhibited highly electrochemical activity and show highly sensitivity for determining of serotonin. (2) We studied the interaction of the serotonin in self-made carbon fiber microelectrodes using CV, DPV and NPA and found the peak currents were linear straightly over the range 4.0 × 10~-6~4.0 × 10~-5 mol·L~-1and 5.0× 10~-6~5.0× 10~-5 mol·L~-1.The electrodes process has been investigated in detail of electrochemistry techniques and its dynamics parameters had been obtained, too.The paper also summarizes the sort, the process mechanism and the development on the scope of Calixarenes and chemical modified electrode. The main work is divided into two chapter. Forstly, We optimize the synthesis technique of p-tert-buthyl calix [6] arene. The structure of production has been characterized by mass, IR, ~1H NMR spectroscopy respectively. A new p-tetra-butyl calix [6] arene-L- Histidine chemical modified glassy carbon electrode (BCH/GCE) has been prepared for simultaneous investigation and measurements of Epinephrine (Ep) and Serotonin (5-HT) by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In potassium dihydrogen phosphate-borax (PDPB) buffer solution (pH 5.8), the anodic peaks of Ep and 5-HT were observed at 0.307V and 0.458V respectively with AE up to 151mV. The peak currents were linear relationship with the concentrations of Ep in the range of 1.0×10~-6~1.30×10~4molL~-1 in the presence of 1.0×10~-4 molL~-1 5-HT on the DP voltammogram. A linear relationship was similarly found for 5-HT in the range of 1.0×10~-6-1.40 ×10~-4 mol L~-1 in the presence of 1.0×10~-4molL~-1 Ep. It is found that Ep and 5-HT could be simultaneously measured with good sensitivity in the presence of 1.0×10~-3molL~-1 ascorbic acid (AA).This method has been applied to the determination of Ep and 5-HT in synthesis samples with satisfactory results. Secondly, Two new amic-calix [4] arenes are have been characterized by IR and ~1HNMR spectroscopy, respectively. The electrochemical method is also used to study the amic-calix [4] arenes. The CV and UV—vis spectroscopy were also be employed for researching the reorganization of metal ion(Cu2+). The results indicate that the two compounds have good ion reorganization character, which provides useful information for application.