Impact And Laws Of NO Effects On Three Algae Growth And Its Application In Red Tide Chemical Forecast

Nitric oxide (NO), as a little, simple compound, is increasingly turning into one of the most hotspot of science research. Due to its broad existence and special actions in the atmosphere and the diversity of its physiological functions in organism and high plants, NO has attracted a great deal of attention from biologist, physiologist, and chemist. NO is entitled "Molecular of year" in 1992. The Nobel Prize of Physiology & Medicine in 1998 is awarded jointly to Robert F. Furchgott, Louis J. Ignarro and Ferid Murad for their discoveries concerning nitric oxide as a signalling molecule in the cardiovascular system. However, no research about NO in oceanological field has been reported. No research about the laws of NO effect on the growth of marine phytoplankton has been reported yet. So on one hand, this paper chooses three algae namely Haeterosigma akashhvo, Chaetoceros curvisetus and Chorella marine to discuss the impact and laws of the NO effect on the growth of marine phytoplankton from chemical point of view for the first time. Firstly, during the cultivation process, nitric oxide of different concentrations is added one or twice a day. Secondly, this paper also discusses the variation of nitric oxide in Chaetoceros curvisetus solution through ISO-NOPMC NO microsensors. And thirdly, set out the NO concentration of algae production and algae optical density, and the relationship between them. The conclusions are as follows:(1) Nitric oxide is added into the medium when the three algae are growing. We found that in f/2, f/50, f/100, f/200 or seawater medium, the growth of these three algae are obviously promoted or inhibited when different concentrations nitric oxide is added one or twice a day during the cultivation process, the result is consistent with the influence of NO on the growth of high plants, and the former result of our colleagues.(2) The NO effect on the growth of marine phytoplankton is kurtosis. Different phytoplankton has different optimum NO concentration. The effect on Haeterosigma akashiwo, and Chaetoceros curvisetus., which is red tide algae, is different from that on the food algae, Chorella marine.â‘ The respond of the red tide algae to Nois more sensitive than that on the food algae; â‘¡the optimum NO concentration on the red tide (10-8~10-9mol/L) is far lower than that on the food alga (10-6mol/L) , which is consistent with the influence of NO on the research of our colleagues.(3) When adding 10-6mol/L NO twice a day, Chaetoceros curvisetus' whole growth curve is like the sidestep. It maybe the results of two kinds of NO threshold concentration.(4) The algae themselves can produce a certain concentration NO. And it maybe have a kind of relationship between the NO concentration and the algae's growth process. As for the Chaetoceros curvisetus and Chorella marine, there always is a NO peak 2-3 days prior to the algae maximum number.The above conclusions show that the effects of different concentration of NO on the growth of marine phytoplankton are very distinctness. These results are the experimental basis of further study on the nitrogen cycle in oceanic ecological system from chemical and biological points of view, which is important to the marine chemistry and marine biogeochemistry. It is of great importance to make use of nitric oxide as the character factor for red tide chemical forecast and its precautionary research.