Technological Research Of Amino Acid Were Extracted From The Waste Brewer's Yeast Cells

This study mainly introduced the research of the receival of six kinds of amino acid from waste beer yeast. It compared deferent treatments with receiving protein from waste beer yeast, optimized technological conditions of the removal of color from yeast extract using active carbon by the orthogonal test. The paper gave out the better technological conditions to separate Phenylalanine, Tyrosine, Glutaminic acid, Histidine, Lysine and Arginine by active carbon absorption, freezing isoelectric point, cation resin. The paper chromatography of amino acid was only one spot.The results obtained were showed as follows:1.The changing temperature antolysis of waste beer yeast was researched and the autolysis were optimized as well, pH6.5, continued about 48h at 50℃ and 16h at 60℃ , yeast cells were treated biologically by autolysis, autolysis plus enzymatic hydrolysis, inducer plus autolysis and enzymatic hydrolysis in order to release yeast cell protein and its decomposition product. The effect of inducer plus autolysis and enzymatichydrolysis was investigated, the result of the rate of lysis was obviously superior to changeable temperature autolysis plus enzymatic hydrolysis and changeless temperature autolysis. A-amino nitrogen can obtained 53mg/gdry-yeast, the dissolved rate of cell protein achieved 91%, the extraction rate of cell ( dry basis) achieved 84%. The free a-amino nitrogen increased 8.0mg/gdry-yeast and 25.0mg/gdry-yeast, the dissolved rate of cell protein increased 15.0% and 31.0%, the extraction rate of cell ( dry basis) increase 7.0% and 23.0%.2.the experimental results of the decolorization of yeast extract using active carbon indicated that: the optimal temperature for decolorization 90°C, the pH 3.0, the dosage of active carbon 1.5g/100ml and the time 35min.3.According to the selective adsorbility, L-Phe and L-Tyr were adsorbed by active carbon from the beer yeast extract, the best active carbon was found, and optimal extract technology were as follows: pH5.8, IN aqueous ammonia, 60% ethanol eluted .4.1n the study on extracting the L-glutaminic acid crystal by the method of freezing isoelectric point, it was found that the best pH value of the mother liquor was 3.22, and temperature for the treatment was OX,.5.Basic amino acids were extracted and purified by 732 cation resin. The optimal method was as follows: using NH+4- cation exchange resin, the concentration of amino acids 9.04g/L, the velocity of flow amino acids...