Effects Of Fluoride Tocixity On Reproductive System Of Male Mouse

[Objectives] Studied on the effects of reproductive of male mice by fluoride with 40^th age,70^th age, 90^th age. 110^th age. Discussed the effects of animal and human in Fluoride District, and that for animal experiment basis.[Methods] Treated 27^th age Wistar mice with 150mg/L NaF water and double-distilled water. Measured the seminal fluid quality, T of blood serum , testicle pathology slices .epidermis growth factor and its receptor in 40^th, 70^th, 90^th, 110^th age .[Results] 1.In fluorine groups, the organs coefficient of testes to body failed extremely remarkable (P<0.01 ) in the 40^th age , but in 70^th and 90^th age, there was no obvious change(P> 0.05 ), the coefficient minished extremely (P<0.01 ) in the 110^th age.2. In fluorine groups, the rate of deformity increased and activity depressed were extremely remarkable (P<0.01 ). Sperm's density was gone, but the dropping was extremely remarkable in 90^th and 110^th age(P<0.01 ).3. Testicle pathology slices showed,the layers of cell were reduced, the thickness of the testes tuble were increased, and the diameters of testes tuble were diminished.4. In determing procession,the layers of cell were extremely reduced and the thicknesses of the testes tuble were minished (P<0.01 ) .The diameters of testes tuble were extremely minished remarkable in 40^th age and 110^th age (P<0.01 ),but the diameters of testes tuble minished were remarkable (P<0.05 ) in the 70^th age.In the 90^th age, the diameters of testes tuble were no obvious variety (P<0.05 ).5. The T of blood serum were aggravated with aged. In fluorine groups, the secretion of T extremely depressed (P<0.01 ) in the 40^th, 70^th, 90^th, 110^th age .6. In the fluorine groups , the expressed quantums of leydig cell ,spermatogonia and spermatocytes were extremely depressed(P<0.01 ) in the 40^th age,,but the spermatid and the junks of testes tuble were no obviously various (P> 0.05 ). The expressed quantums of leydig cell ,spermatogonia , spermatocytes , spermatid and the junk of testes tuble were no obvious difference (P> 0.05 ) in 70th ,90 th,110 th age.7. The expressed quantums of all measured targets were lessened in 40^th age,70^th age and 90^th age,but for spermatid were extremely knocked down in 40^th age and 70^th age(P<0.01 ). the junks of testes tuble were obviously depressed in 90^th age(P<0.01 ). In 110^th age,all targets were no obvious change (P> 0.05 ),but the quantums were increaced. The junks of testes tuble were added in the 110^th age (P<0.05 )[Conclusions] Excessive fluoride injures testes tissue which can go into blood tesiis Protective Screen. So the sperm can be deformed and no activity . And excessive fluoride can harm the sperm.Meamwhile it can effect the level of T which testes secret, it can impact on the EGF and EGFR which can regubte the function of testes and the measurement of sperm.