Studies On Solid State Culture Of Phanerochaete Chrysosporium And It's Bioremediation To Soil Contaminateded By PCP

The solid state culture including temperature and PH ect of Phanerochaete chrysosporium, a typical white rot fungus, and its bioremediation to PCP contaminated soil were studied. In addition, organic matter types, soil water content and temperature which determined its inhabitionin soil were also examined. Our results are as follows:The CFU of Phanerochaete chrysosporium reach the peak when incubate 7 days at 37℃ using the mixture of potato+sugar liquid and sawdust, keeping the initial water content 60% and PH 6.5. Under such optimal conditions, the LiP and MnP activity reach the highest level at 10th 12th day respectively. Culture in PVP poke can be used as the best method to produce in bulk.Remarkable influence of drying methods and time on water content and spore content was observed. 2% spore and 49% water content were lost under natural temperature for 48 hours. Freezing drying is the most effective approach to decrease water content. 6% spore and 53% water content disappeared after 16 hours treatment. Because the essential factor of fungus agent is the number of living spore, we can select the room temperature drying for long time or freezing drying for short time according to time requirement.The best inhabition conditions in soil of Phanerochaete chrysosporium is: straw addition being as the main nutrition, maintaining 25% water content and 28℃-35℃.The water content and temperature is more important than instant organic matter nutrition.we bioremediate the PCP contaminated soil on site through fungus application. After six weeks, The initial PCP concentration of 20.1mg/kg was degraded to 12.0mg/kg. The degration ratio was 40%. The initial PCP concentration of 34.84mg/kg and 58.71 mg/kg, the degration ratio was 26.5% and 21.1% respectively. Phanerochaete chrysosporium had higher degradation to low PCP concentration.The application of Phanerochaete chrysosporium to soil produce great influence on soil borne microorganism and enzyme activity. The number of bacteria decline at the beginning, and then increased. The number of fungi and actinomyces always higher than that of the contrast. Multiphenolic oxidase activity enhanced greatly, proteinase activity always showed higher than that of the contrast, but the urease activity has little change.