Study On Hydrogen Production By Bacteria

Biotechnology of hydrogen production by fermentation (BHPF), one of the key pathways for environmental pollution control and clean energy acquirement, has been attracted more and more attention all over the world. In this paper, to investigate the culture of hydrogen-producing bacteria and the effects of organic acids and pH value on fermentative hydrogen production from biomass, under anaerobic conditions, at 37 ℃ and by using batch fermentative devices designed by ourselves, some experiments concerned were carried out with anaerobic digestive sludge as inoculums of hydrogen-producing bacteria.By the way of optimized screening. 150 strains of fermentation bacteria were isolated from sullage. As a result, only 12 strains of fermentation bacteria produced hydrogen, and the cumulative hydrogen production potential was low. It was found that the fermentation type was ethanol-type.The substrate containing glucose was treated with natural anaerobic microorganism from sullage. The biohydrogen was produced through anaerobic hydrogen fermentation. Under the experimental conditions, the maximum hydrogen productivity was 2.1mol/mol (H2/glucose) under the conditions of optimal initial pH value of 5.5. The optimum concentration of glucose was 5~30g/L. The optimum substrates were glucose, xylose and starch. The...