Modeling Of Dye-ligand Affinity Chromatography And Flowthrough Chromatography

The mathematical modeling of chromatography is of particular interest tobioengineers, since it can be used as a useful guide and powerful tool in the optimaldesign of the operation scheme or scale up of a chromatography process. The followingwork has been done about theories for dye-ligand affinity chromatography andflowthrough chromatography:A mathematical model for dye-ligand linear-gradient chromatography of a binaryprotein system was established based on the axial dispersion theory and pore diffusionmodel. Bovine serum albumin (BSA) and hemoglobin (Hb) were used as model proteinsand Cibacron blue 3GA (CB) modified Sepharose CL-6B (CB-Sepharose) was used asadsorbent. The binary adsorption isotherms of the proteins were expressed by the stericmass-action (SMA) formulism. It was found that the model predictions agreed reasonablywell with the experimental data obtained under various conditions. It indicates that thebinary SMA formulism was useful for simulation of nonlinear chromatography.A mathematical model for flowthrough (perfusion) chromatography, namelypacked-microtubes (PMT) model, has been proposed for a column packed with biporousanion exchanger in which the micropores and flowthrough pores are created with liquidand solid porogens, respectively. The model is established based on the assumption thatthe biporous particle is made up of packed microtubes. Three kinds of anion exchangers(i.e., microporous, macroporous and biporous resins) are prepared and were used asadsorbents to determine the model parameters and to evaluate the model. It is found thatthe model predictions agree reasonably well with the experimental data obtained undervarious conditions, which suggests that the structure assumed in this model is reasonable.