| Red globe grape, produce American California at first, belong to Eurasianspecies. Because its fruit grain is large, the color and luster is gorgeous, pulp isrigid and friable, the dissolubility solid content is high, the yield is high, the fruitis not rip, the fruit brush embed interior of the fruitery is thick and long, don't takeoff the grain, bear the hoarding, the shelf life expect is long, the red globe grape isdeeply welcome by people. But the anti-disease and anti-medicine ability of redglobe grape are bad, how to prolong the purveying time and keep its good quality,have been the very problem which the planter and researcher pay attention to. Atthe same time, the non-pulluted food has been recognized by the people anddeveloping the green storage of the non–pollu ted grape has been one of the maindirections that the grape protects the fresh technique research.The propolis is a kind of inartifical material, not only implying varioushelpful biologic active composition to human body, but also taking on greatefficiently and widely unbactrium function. Propolis will become the very thinfilm of a layer in the food surface by daubing which can insulate the oxygen,separate the microorganism and reduce the function of the humidity evaporation,lower the breath strength and metabolisms, reduce the depletion of the nutrient,thus prolong the time of protecting fresh, keep its corruption from change incharacter.The HACCP ( the Hazard Analysis and Critical Control Point) is amanagement system, which has greatly prevented function, used to protectingfood, preventing from the biologic, chemistry, physics bane.This text study the fresh technique of the red globe grape without the harmfuleffects, adopting three kinds of methods coalecented: low temperature, inartificalpreservative and system of HACCP.The main work and conclusion are as follows:1) Follow general principle which is to choose the factor and levels as far aspossible little, has the prominent influence to the red globe grape to protect thefresh, make sure the propolis density and the temperature of storage and degree ofhumidity three experiment factor, carrying on three factors, three levelsesorthogonal array.The result enunciated that the biggest influence to protect thefresh of the red globe grape is the propolis density A, showing the prominent level0.01;the next factor is temperature B, showing the prominent level 0.05;then isthe degree of humidity C, showing the unobviously influence. The superiorcombination is an A2B1C2, namely 15% propolis density, store in 0.5 ℃, degree ofhumidity 85%～95%. The method under that condition is the best one to protectthe fresh.2) Falled grain rate, putrefied rate and lost gravity rate present the up-trendduring the period of hoarding, the dissolubility solid content presents thedescending trend. The descending range is obvious low to the contrast set. Theactivity of SOD enzyme;the content of MDA, the POD enzyme activity, thebreath strength appear descending after a most high peak, which means the abilityof clearing live oxygen inside the body descending and the oxygenation with thefilm fat function strengthening.3) The length of the storing period and the quality of the grape are directlyinfluenced by the bee propolis density. The film thickness is different because ofthe different bee propolis density. Too thin film is ineffective to form film and theintegrity of the film, which can't become the high CO2 availably with the smallenvironment of the low O2 and exertive this small environment of function.4) Breath strength maintenance is in the lower level at the 30 d and the SODactivity is higher at this time, expressing that the ability of the fruit to remove thesuper oxygen anion free radicals is strong, thus can reduce the harm of the film.The content of MDA is low as the outcome of the oxygenation with the film fatfunction, but the MDA can alsocreat by the non-enzyme function, the repressingmeasure of enzyme is not greatly to, so the MDA content of the manage samplehas been presenting the slow-moving rising appearance after 15 d. The variety ofthe POD activity is also basic consistently ( present the clock cover form in 30d).The activity of pectic enzyme keeps higher to went with at 30d for the low leveland the fruit degree of hardnesses at the 30d. The SOD enzyme of the highactivity, is the performance of the anti-decrepitude. The high activity of the SODenzyme can prevent from the activity of the oxide enzyme, the oxide enzyme togo up, and restrain the biologic film to be oxidized effectively. Relevant fewenzyme activities and correlation should be treated in further research.5) Seven basic principles which followed by the implement HACCP systemare be elaborated. The character of red globe grape is descripted which is theproduct of the implement HACCP system. Make sure the hoarding craft of the redglobe grape and carried on the elucidation. Analize the hazards to the hoardingcraft of red globe grape according to the basic principle and methods of theHACCP system and put forward the control measure.Made sure 11 key controlpoints according to the analysis tree of key control point, and build up work formof analizing hazards.6) Build up the control limit value, monitor the procedure, the keepingrecords, rectify measure, verification the procedure and the managing procedurewith file of the HACCP plan to the craft of the red globe grape. Draw up theHACCP work plan form.Put forward the method, usage faintness synthesizes toadjudicate the sense organs quantity of the red globe grape, to adjudicate validityof the implement HACCP system to the red globe hoarding craft.7) At the foundation of making use of the covariance method to certain thepower heavy of each factor, make use of the faintness to synthesize to adjudicatethe sense organs quality of red globe grape, which store 100 days under differentcondition. Avoid the influence that factor bring with factitiousness and subjective.Thus acquire an overall, fixed amount synthesized adjudication system.8) Use the statisticses method to make sure the synthesized adjudicatedpower value of the color and luster, taste, eating and the appearance of the fruitpedicel is: A=(0.2308,0.2895,0.3215,0.1582).9) Built up the adjudicatd factor set U and comments set V. Throughprocessing the sense organs quality assessed result, we acquired the synthesizedadjudicated result, carry on unitary processing and the adjudicate result is:G1 set: W1 =88.82(good) W 2=88.71(good) W 4=93.60(excellent)W5 =90.58(excellent) W7 =88.41(good) W8 =87.91(good)G2 set: W1 0=87.07(good) W 20=88.43(good) W 40=91.12(excellent)W5 0=87.90(good) W7 0=87.89( good) W8 0=86.60( good)Line up the preface result:G1 set: W 4> W5 > W1 > W 2> W7 > W8G2 set: W 40> W 20> W5 0> W7 0> W1 0> W8 0Under the hoarding condition of low temperature, the sense organs quantityof all samples is comeing to a good above. And the samples 4 results of two setsare all the best, explained the sample 4 of hoarding condition is the bestcombination, namely: 15% propolis density, store in 0.5 ℃, the degree ofhumidity is in 85%～95%.10) The sense organs quantity of the each sample of G1 set ascend the G2 set,can explain the red globe grape hoarding to protect fresh process is valid byimplement HACCP system. From the row preface result, we can see two rows ofsample preface result is different, and it can explain that red globe grape hoardingand carrying out the HACCP system not only have the good result on wholely inthe fresh process, but also the function of the HACCP system and bee propolis iscombined together, which can change the function result of the bee propolis on acertain degree.The innovation points of the thesis:1) Choose the propolis as preservative to study the non-polluted hoarding ofthe red globe grape;summarized the best density of propolis.2) Implying the HACCP system to the hoarding craft of grape , carrying onthe hazard analysis;summatized the critical control point.3) Imply the faintful synthesized adjudication to the sense organs quantity ofthe red globe grape and accord to the result to adjudicate the validity of theHACCP system.
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