Effects Of Temperature And Copper On Serum HSP70 Expression Of Carp (Cyprinus Carpio)

To determine the effects of temperature and copper on carp fishs (Cyprinus carpio)serum HSP70,we design four different plans.. HSP70 expression relative to the controls wasanalyzed by sandwish ELISA (Enzyme-Linked Immunosorbnent Assay ELISA);Copperconcentrations in fish serum were measured by atomic absorption spectroscopy (AAS). In thedesign of increasing temperature step by step , the results showed that ,with the temperatureincreasing , serum HSP70 increase gradually;In the design of increasing temperaturesuddenly , the results showed dose–response effects of HSP70 expression in carp serum under10℃;The HSP70 expression in carp serum under 20℃,24℃ are both much higher than thatunder 10℃(P<0.01). We observed no combined effects of temperature and copper on HSP70expression under 20℃;With the increase of copper dose , the expression of HSP70 did notchange much compared to that of control group . However , there was strong combinedeffects of temperature and copper on HSP70 expression under 24℃. The HSP70 in carpserum under 100ug Cu2+/l was much higher than that of control group (p<0.01) while thatunder 300ugCu2+/l and 500ugCu2+/l was lower than control group on different scales,respectively. The results of this study demonstrate that the HSP70 induction in carp serumwas sensitive compared to TL50,LC50 . Furthermore , we concluded that heat shock was themain HSP70 inducer and under different temperature , copper has different effects on HSP70expression . HSP70 is the suitable marker to reflect the disadvantageous ecological stress .