Researches On Metal And Organic Compound Resistances Of Acidithiobacillus Ferrooxidans Isolated From Chinese Mining Areas

Biohydrometallurgy develops on the basis of hydrometallurgy, which values the use of the metabolism of microorganism or their products. Compared with traditional smelting technologies, biotechnology has the advantages of lower energy and financial expenses, operating simply, making fully use of resource, no pollution process. Moreover, it can be applied to treat refractory or low-grade ores, which challenges traditional technologies. Nowadays, a large quantity of bioleaching operations has been applied all over the world. At.f is an important bacterium in the course of leaching;it can oxidize ferrous irons and reductive sulfur and results in metal ions leached.We isolate and purify sixteen strains of At.f from the acid mine drainage. Firstly, we determine the degree of resistance of different strains of T. ferrooxidans on Ni²⁺,Cu²⁺, Mg²⁺ , Co²⁺, As³⁺, As⁵⁺, Hg²⁺. We could domesticate the selected proper candidates based on their resistant characteristics to make them most efficient to different types of Chinese mines. Secondly, we examine the inhibitory concentration variety of organic compounds, glucose , xylose, citric acid, cellobiose, TSB, to develop a possible preventive treatment of AMD. On purpose, we want to explore the possibility of applying natural substances to prevent the formation of AMD before and in the course of exploitation of various deposits in Chinese mining areas economically and environmentally. The plasmid profile of At.f of different Chinese mining areas was examined in order to screen proper gene vector, which were discussed relating to the mechanism of their metal and organic compound resistance character.