Fundamental Apply Study On Degrating Nitro-Compound Of White-Rot Fungi

The dissertation studied the enzymatic properties of manganese peroxidase (MnP) , MnP degraded aniline and nitrobenzenes, white-rot fungi biochemically treat diazodinitrophenol (DDNP) waste water which had been treated after microelectrolysis. Organic pollutants when were degraded need not enter white-rot fungi cellular. Toxic materials can not easily attack white-rot fungi. White-rot fungi also can degrade insoluble compounds.The mycelia were screened by asynchronous culture, then cultivated mycelia through third-order enlarged methods. Not only microelectrolysis, but also did the process of biochemical need to invest much, materials are obtained easily, and achieved the purpose of using waste control waste really.Shaking culture and static culture were explored about MnP enzymatic properties. We explored effects of quantity of microelementand and nitrogen source, pH, temperature, liquid volume in flask, Mn²⁺ concentration affecting, amount of inoculums, nitrogen source, and carbon source to MnP activity. MnP activity was high in shaking culture when ZnSO₄·7H₂O was 0.03g/L, MnSO₄-H₂O was 0.0017g/L, C₄H₁₂N₂O₆ was 2.0mmol/L When pH=4.5, MnP activity was well in increment and survival time. Survival time was 17h When pH=4.5. When temperature was 37℃, MnP activity was better then temperature was 20℃ and 50℃. The liquid volume in triangular flask was 30ml/250ml in static culture which MnP activity was well. But in shaking culture, the liquid volume in triangular flask was 50ml/250ml. When Mn²⁺ concentration was 2mg/L, MnP activity was well in shaking culture and static culture. MnP activity was well when 1aperture/flask in static culture, 2aperture/flask in Shaking culture, nitrogen source was C₄H₁₂N₂O₆ , and carbon source was glucose.In degradation experiment of aniline and nitrobenzenes, the relationship of reaction velocity vs. Enzymes contained was first-order reaction kinetics relationship. Vm and Km of aniline and nitrobenzenes were confirmed. (aniline:Vm=0.2985mg/L·min, Km=2.8692mg/L;nitrobenzenes:Vm=0.8325mg/L·min, Km=0.7525mg/L)Adding 5%-8% peat was valuable to improve biochemical effect in enlarge experiment And the drainage of aniline and nitrobenzenes were attained to the third level of 《the second sort pollutants allowance emissions》. The pH of drainage waste water was approximate 7.