Study On The New Methods For The Determination Of Some Pharmaceuticals By Spectrophotometry And Resonance Rayleigh Scattering

Spectrophotometry is a classical, rapid, simple and convenient analytical technology, which is been widely used to analyze pharmaceuticals. Resonance Rayleigh scattering is a new analytical technology developed in recent ten years. For its' excellence of high sensitivity, cheapness instrument, simple operation and rapid analysis speed. At present, the method bring to more attention and interesting with its' applied in determining pharmaceuticals more and more. So we used the two analytical technology in studying sodium hyaluronate, betahistine hydrochlonide sodium chloride and patassium dehydroandrographolide succinate.This dissertation has studied the applications of spectrophotometry and Resonance Rayleigh scattering method in some pharmaceutical analysis. 1. some basic bisphenylnaphthylmethane dyes- sodium hyaluronate systems. 2. coomassie brilliant blue G250 - betahistine hydrochlonide sodium chloride system. 3. some basic triphenylmethane dyes- patassium dehydroandrographolide succinate(PDS) systems. Their A(absprption) and RRS(Resonance Rayleigh scattering) spectral characteristics, optimum reaction conditions, influencing factors and analytical applications have been studied. Furthermore, the reaction mechanism, the reasons of A or RRS changedand the relationships between RRS spectra and absorption spectra are discussed. The main research contents are as follows:1. basic bisphenylnaphthylmethane dyes- sodium hyaluronate systemsIn weak acid medium, bisphenylnaphthylmethane dyes such as night blue (NB), Victoria blue B (VBB) and Victoria blue 4R (VB4R) , react rapidly with Sodium Hyaluronate to form complexes, which causes the change of absorption spectra, the decrease of absorbahce near 612nm(NB),616nm(VBB),585nm(VB4R) separately, and AA(=A-Ao) values are proportional to the concentration of Sodium Hyaluronate, Beer's law is obeyed in the range between 0.5-2.0 mg/L(NB) ,0.1~2.5mg/L(VBB) and 0.1-0.6 mg/L(VB4R) respectively for Sodium Hyaluronate. The molar absorptivities of different systems are between 1.51 X108- 5.52 X108 (the molecule of SH is 5 X105). Based on these, we developed a new spectrophotometric method for the determination of Sodium Hyaluronate. The method is simple, rapid, repeatable, and of good selectivity, and has been used for the determination of total amounts of Sodium Hyaluronate in samples with satisfactory results.In weak acid medium, basic bisphenylnaphthylmethane dyes such as night blue (NB), Victoria blue B(VBB),Victoria blue 4R(VB4R) reacts rapidly with Sodium Hyaluronate to form complexes, which results in great enhancement of resonance Rayleigh scattering(RRS) and a new RRS spectrum. The maximum RRS peak is located at 408 nm(NB), 285.5 nm(VBB), 276.5 nm(VB4R) respectively. RRS intensity is proportional to the concentration of sodium hyaluronate in the range of 0~1.5mg/L(NB),0~5.0mg/L(VBB), 0~3.0mg/L(VB4R) separately. The method has high sensitivity and the detect limit (3 o) for hyaluronic acid is 13.7ng/mL(NB), 14.9ng/L(VBB), 22.1ng/L(VB4R) respectively. The method also has good selectivity, and has been used for the determination of total amounts of sodium hyaluronate in samples with satisfactory results.2. coomassie brilliant blue G250 - Betahistine Hydrochlonide SodiumChloride systemIn this paper, the spectra characteristics of absorption of the BetahistineHydrochlonide SodiumChloride- Coomassie Brilliant Blue G250 system, the optimum conditions of the reaction and some parameters of standard curve have been investigated. Based on this, We build a new method to determine the Betahistine Hydrochlonide SodiumChloride in its injection solution.3. some basic triphenymethane dyes - patassium dehydroandrographolidesuccinate (PDS) systemIn weak acid medium, some basic triphenylmethane dyes, such as MV, EV and CV react rapidly with PDS to form complexes, which results in great change of absorption and a new absorption spectrum. The maximum Abs peak is located at 606 run (EV) . AA intensity is proportional to the concentration of PDS in the range of 0.2~8.2mg/L (EV). A new method for the determination of PDS has been established on the basis of the above mentioned phenomenon. The method has high sensitivity and the detect limit (3 o ) for PDS is 23ng/mL. The method also has good selectivity, and has been used for the determination of PDS in its injection solution samples with satisfactory results.In pH 4.4 Britton-Robinson butter solution, some basic triphenylmethane dyes, such as MV, EV and CV react rapidly with PDS to form complexes, which results in great enhancement of resonance Rayleigh scattering(RRS) and a new RRS spectrum. The maximum RRS peak is near 650.5nm. RRS intensity is proportional to the concentration of PDS in the range of 2~52mg/L(MV))2~50mg/L(CV), 2~70mg/L(EV) separately. The method has high sensitivity and the detect limit (3 o ) for PDS is 13.9 ng/mL(EV), 28.9ng/mL(MB), 23.5ng/mL(CV) respectively. The method also has good selectivity, and has been used for the determination of total amounts of PDS in samples with satisfactory results.