Study On Technique And Application Of Capillary Electrophoresis With Amperometric Detection

Capillary Electrophoresis (CE) is one of the most important and rapidly growing separation techniques in recent twenty years, It absorbed the virtues of both the conventional electrophoresis and modern High Performance Liquid Chromatography (HPLC). However, the theoretical plate number is great developed to tens of thousands and even millions and the volume of sample injection reaches nano milliliter level in CE analysi. In recent years, capillary electrophoresis (CE) has been extensively studied and applied as a highly effective analytical method in modern analysis. And excellent prospects of CE have been shown in the fields of pharmaceutical analysis, biochemical analysis, food analysis, and environmental analysis. It has many advantages such as much lower sampling volume, cheaper instrumentation and higher separation efficiency. However, the small sample injection volume and very thin capillary bring about difficulties to detections. The common used detection methods are Ultra-violet (UV), Laser Induced Fluorescence (LIF),Luminescence Detection, Mass Spectra (MS). Because amperometric detector (AD) is more sensitive than UV-visible detector and much cheaper than LIF detector, it is preferred to couple with CE in many analysis areas.The Goal of this dissertation is to apply this CE-AD technique to resolve some actual analytical problems in pharmaceutical analysis, environmental analysis. The contents of the dissertation include four chapters:In the first chapter, the characteristics of CE, the separation models and basictheories of CE, the detectors, the injection technologies of CE,the studies and applications of CE-AD simply is introduced.Chapter 2 is the determination of aminoheterocycle and azabicycle in gliclazide bulk by capillary zone electrophoresis with amperometric detection. A simple, reliable...