The Formation And The Kinetics Of The Nanoreactors

This dissertation focuses on the formation and kinetics of nanoreactors based on the SBA-15 mesoporous materials and porcine pancreatic lipase (PPL), utilizing the hydrolysis action of olive oil as the model action.1. The pore diameter of the SBA-15 as hosts for enzyme immobilization has significant effects on the internal diffusion under a negligible external diffusion resistance condition. The general problem of substrate transport in nanometer confinements is involved. At smaller pore size (R<9A run), the internal diffusion limitation significantly occurred and the process is diffusion rate controlled. But the diffusion limitation plays inconsiderable roles at larger pore diameter (R>10.9 nm).2. SBA-15 hosts were grafted with three kinds of functional alkoxysilanes (PD, MA and DE) and the derivates were employed as the hosts of immobilized enzymes. The nanoreactor grafted by PD exhibits amphiphilicity, hence highest activity and facile accessibility of H₂O and...