| Paper industry is one of the industries that cause serious environmental pollution. The key of paper industry sewage treatment is the efficient treatment of lignin. By a host of experiments, On the basis of exploration of new assistant reagent and its effect for paper industry black liquor, a detailed study had been done on separation of efficient lignin-degrading fungi, their growth, reproduction, lignin-degrading capacity, and synthetic experiments of bioaugmentation factors and practical simulated experiments, which offer theoretical basis to improve removal effect of lignin in paper industry black liquor.1 Seven fungi have been separated from 51 strains, which grew well on the culture media contained lignin.A preliminary study has been carried out on the capacity of lignin degradation of the strains.2 The generation time is parting for strain A for 17.1h,strain B for 14.6h,strain C for 22.4h,strain D for 19.3h,strain E for 27.4h,strain F for 20.3h,strain G for 9.6h of versus chaffer strain growth behavioural study.3 The practical situation of warranty papermaking vocation black liquor waste water effluent,experiment condition introduction be intended to be temperature 30℃, pH for 8 of the both in vitro,experimental result proclaim strain in order growth temperature and pH experimental result for strain A for 30℃, pH for 7,strain B for30℃, pH for 7,strain C for30℃, pH for 7,strain D for 30℃, pH for 7,strain E for 30℃, pH for 8.5,strain F for 35℃, pH for 7,strain G for 35℃, pH for 7.5.4 Being on the experimental foundation,the degradation effect upper strain C, D, E is selected for experiment strains .Adopt induction of mutation law-ultraviolet irradiation mutagenic law and tilize fish out appropriate mutagen quantity,A train of mutagenize is design to lignin degradation strain. By intermediate culture,the growth and degradation effect preferable strain X, Y, Z is acquired. Strain degradation effect mensuration with the result that lignin degradation property have got overt advance, strain X degradation property is better above strain Y, Z.5 Through the medium of growth curve ascertain mutagenic strain generation time strain X for 13.9h,strain Y for 15.6h,strain Z for 9.2h, and compare with experiment strain generation time have got shorten.6 The highly active strain characteristic proceed for detailed study,on prime different kinds of ambient conditionand,strain growth and bred,degradation lignin change proceed clearly experimental study,fish out know clearly optimal strain culture condition,at the some condition simultaneous existence combine action,strain degradation lignin efficiency get smart advance,maxima lignin removal rate approve to 40% upwards up in of the bisection separate out. the conditional experimental study proven,act as temperature for 30℃, the growth and bred of the pH for 8. 120r / min shake culture ,be advantage for strain.7 Through the medium of manifold cometabolism primary carbon source and nitrogen source efficiency comare,ascertain glucose for appropriate cometabolism primary carbon source,ammonium nitrate for appropriate nitrogen source;at culture medium inorganic ions append and addition perpendicular experimental study proven,to inhere degradation culture join join 10mg/L Mn2+ ion,0.1 mg/L Cu2+ ion,0.25mg/L Fe2+ ion,met far advance lignin removal rate;syntheses condition perpendicular experimental study proven,culture medium of cometabolism primary carbon source glucose strength for 2g/L,ammonium nitrate strength for 1.5g/L, culture scale for x:y:z for 1:1:1,The condition lignin removal rate is most for ideal.
|
PDF Full Text Request