The Preparation Of Monolithic Columns For Capillary Electrochromatography And The Study Of Application Of Chromatography Methodology

This thesis includes two parts: Part 1 has three chapters (chapter 1 ,2 and 3) and Part 2 has two chapters (chapter4 and 5).Part1 The preparation of monolithic columns for capillary electrochromatographyIn chapter 1, a review on the recent advances in capillary electrochromatography was presented.In chapter 2,a novel approach has been first introduced and evaluated for the preparation of silica-based monoliths in the application of Sol-gel technologies, which in situ polymerization was carried out by γ-ray irradiation within the capillary. It provided a viable alternative to either thermally initiated or photo polymerization.In chapter 3,the rapid preparation of monolithic columns for capillary electrochromatography separation by UV-initiated polymerization and Sol-Gel technology has been first introduced and evaluated. The chromatographic behavior of eight PSG columns were comparatively studied, all of which exhibited reversed-phase character. Using these columns, a variety of neutral compounds including thiourea, benzene, toluene, ethyl benzene, biphenyl and naphthalene were separated from mixtures.