Study And Application Of Determination Method Of Trace Copper (Ⅱ) By The Catalytic Kinetic Spectrophotometric Analysis

Copper is considered as an essential micronutrient for animals, plants and human beings. And this element is also considered as an ambient contaminant in the environment. Some conventional determination methods of copper (II) in seawater can be applied only after preliminary treatment and pre-concentration, and they need expensive instruments and reagents. In recent years,the analysis method based on catalyzed kinetic reaction has been developed and been proved to be an attractive means for trace analysis.In this paper a new analysis method is established for determination of trace copper (II) based on the catalytic kinetic theory. The mechanistic of the reaction is studied, the copper in fresh water and sea water are determined with the new method, and then the characteristics for sorption of copper (II) on the sediments of Jiaozhou Bay (Dagang area) are studied with the new method. The main results of the research are as follows:1. A new catalytic kinetic spectrophotometric method is established for the determination of trace copper (II).In this study a new method is established for the determination of copper (II) based on its catalytic effect on the oxidation of glutathione by potassium hexacyanoferrate (III) in acidic medium at 25.0℃. The results from the orthogonal experiments and the single experiments show that the optimal experimental conditions are as following: 1.50×10-3 mol/L K3Fe(CN)6, 9.00×10-3 mol/L GSH, pH = 4.00, the reaction time is 5.00 min and the temperature is 25.0℃. Under the optimum conditions, the proposed method allows the determination of copper (II) in the range 0～35.0 ng ? mL?1 and the detection limit is down to 0.04 ng ?mL?1. The recovery...