Technology Of Capillary Electrophoresis With Electrochemical Detection In Pharmaceutical Analysis

Chapter 1 PrefaceThe preface consists of three parts. The first part describes the role and the trend of pharmaceutical analysis. In the second part, the principle, the characteristic and several types of detectors of CE technology is simply introduced. The second part emphasizes the application of CE technology in many analytical fields such as small molecules detection, DNA analysis, protein detection, drug quality control and clinical detection. It also discusses the new trend of the development of CE technology in both application and equipment aspects. In the third part of the preface, the intention and innovation of this dissertation are summarized. This review covers 133 references.Chapter 2 Determination of amiloride hydrochloride and hydrochlorothiazide in pharmaceutical preparations and spiked human urine sample by CE-EDCompound amiloride hydrochloride tablets are applied to the therapy of hypertension. A high-performance capillary electrophoresis with electrochemical detection method was developed for the determination of amiloride hydrochloride and hydrochlorothiazide in tablets and spiked human urine sample. Relevant parameters of the separation and electrochemical detection were examined and optimized. Under the optimum conditions, the two analytes could be well separated within 8 min at the separation voltage of 18kV in a 40mmolL^-1 running phosphate buffer (pH8.0). A carbon disk working electrode, applying +1.0V(vs. Ag/AgCl, 3M KCl) as detection potential, was used to detect the two eletroactive analytes. The linear ranges were from 0.40 to 20μgmL^-1 for amiloride hydrochloride and 0.30 to 131μgmL^-1 for hydrochlorothiazide, with correlation coefficients of 0.9983 and 0.9990,respectively. The limits of detection of the two analytes were 0.08 and 0.10μgmL^-1 respectively. The recoveries of the tablets samples were between 100% and 106%. The two standard analytes of certain concentration were spiked into blank human urine and...