Spectrophotometric And Electrochemical Studies On Glycosaminoglycans

Glycosaminoglycans (GAGs) are most important component in all lives of the world, which play significant roles in the research fields of life science, chemistry and medicine as information molecular. Spectrophotometric and electrochemical methods have many advantages such as cheaper apparatus, simplicity of operation and high sensitivity, so they were widely used in the research of the interaction between biomolecular and organic molecular. In this thesis, heparin is selected as model of GAGs for the investigation, which has no spectrophotometric and electrochemical responses itself. Several organic dyes are selected as probes for the studies on the interaction with heparin, which can interact with heparin by electrostatic force to form a supramolecular complex, and result in the changes of spectrophotometric and electrochemical response. The changes of absorbance or currents are furthur used to establish sensitive analytical methods for the determination of heparin.The paper can be summarized as follows:1. The spectrophotometric method for heparin are established using neutral red(NR) and acridine orange(AO) as spectrophotometric probe. NR and AO has specific absorption spectra in the wavelength from 300 to 700 nm. After the addition of heparin, the absorbance value decreased greatly, which indicated that an interaction had taken place in the mixed solution. The conditions of the binding reaction were optimized and the influence of some coexisting substances such as metal ions and amion acids were investigated. Under the optimal condition, the working curve of the heparin was established and the decrease of absorbance at maximum absorption peak was in linear...