Enzymatic Synthesis Of L-ascorbyl Palmitate And Glyceryl Linolenic Acid

Over the past decade,non-aqueous enzymology has emerged as a major area of research and development in biotechnology.In order to exploit non-aqueous enzymology fully,the work studied the various factors which affect the conversion of reactant of ester synthesis reaction in non-aqueous solvents. The main contents are as follows:1. With aspergillus niger as catalyst,preferred conditions for synthesis of L-ascorbyl palmitate from methyl palmitate and L-ascorbic acid were studied in detail. Results show that, when methyl palmitate and L-ascorbic acid was mixed at the molar ratio of 1.3:1.0, with dosage of 15% methyl palmitate as catalyst, under 36℃, under reaction for 24h,L-ascorbyl palmitate was obtained with a yield of 23%.2. The enzymatic esterifications of glycerol with linolenic acid were carried out efficiently using Novozym 435 in solvent-free system. When glycerol reacted with linolenic acid at 60℃, with a molar ratio of 2:1, catalyzed by 1% weight of lipase to linolenic acid, the maximum conversion of linolenic acid received 90%.3. The enzymatic esterifications of glycerol with linolenic acid were carried out efficiently using Novozym 435 in organic solvent system. When glycerol reacted with linolenic acid at 60℃, with a molar ratio of 2.5:1, the maximum conversion of linolenic acid received 90%.The conversion of linolenic acid had no difference in free-solvent and organic solvent,but the products were different in two systems.For the former, triacylglycerol was the primary product ; for the latter,however,mono- and diacylglycerol were the primary products.