Studies On Fermentation Techniques Of Nosiheptide

In order to optimize the fermentation conditions of Streptomyces actuosus for the industrial production of nosiheptide, Streptomyces actuosus and its fermentation techniques were studied in this paper,Firstly, the strain YM4-2 was isolated from parents strain of Streptomyces actuosus, after selection the isolation medium and seed medium were determined Then, the extraction techniques and the bioassay method, the TLC-UV method the TLC-bio-autography method and HPLC method were developed in order to detect nosiheptide and related materials in the fermentation broth The optimized fermentation conditions of Streptomyces actuosus for the production of nosiheptide were as follows: fermentation tempreture, 28-30 ℃; fermentation period, 4-5d; seed age, 27h; inoculation volume, 2.5 -5.0%(v/v); fermentation volume, 30ml in 250ml shaking flask; pH, 6.6-7.5 before sterilization The fermentation media for nosiheptide production were screened and optimized by uniform design in this papero The optimal carbon source and nitrogen source were starch and soybean powder respectively through the study, and the metabolized curve was obtained under the optimized fermentation conditions The nosiheptide titer increased obviously by supplying nourishment to fermentation medium during the fermentation procedures Feeding amino acids to fermentation medium from 5mM to 15mM at the beginning of fermentation was performed in this paper., Ser, Glu Glu-Na...