Mechanism Study Of A Strain By Protoplast Fusion Application Microbial Profile Modification

It is one of the pivotal technology to select excellent performance a strain in MEOR. It will influence on the effect of application in oil field and developmental foreground in MEOR.In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion. The methods of biochemistry experiment, laboratory physical simulation experiment, micro-imitated glass model and environment scanning electron microscope (ESEM) compared the physiology and biochemistry characteristic and the growing condition of the strain adapting oil formation of the bacterium FH, which obtained by the way of protoplast fusion, to the bacterium JD, which is one of parents strains, selected the culture medium of the bacterium FH, analyzed the transport and shut-off capacity of the bacterium FH in porous media, and recovery efficiency of water driving oil in nonhomogeneous formation, studied the profile modification and oil displacement mechanism. Furthermore, some systematic conclusions were also acquired.The results show that the bacterium FH can metabolize in oil formation rather than one of parents strains, which notated as bacterium JD, show that the microbial polysaccharide, which bacterium FH producing in porous media, commendably can close off big channel, can adjust injection profile, can enhance recovery efficiency of water driving oil in nonhomogeneous formation. The experiment by the way of micro-visual analysis with micro-imitated glass model and environment scanning electron microscope (ESEM) indicated the bacterium FH can produce microbial polysaccharide and microbial gas in porous media. Some polysaccharide is in the water phase, some adsorb surface of rock grain with some thallus bring on reducing the pore radius. Movable microbial gas can be capable of drive oil, and immovable...