Effects Of Heavy Metal Ions On Toxicity Of Charybdis Japonica

Recently, environmental pollution along coastal areas in China is serious increasing, expecially heavy metal contamination in sediments, for example that mercury (Hg) concentration in Jinzhou Bay, Hg, cadmium (Cd) and copper (Cu) concentration in estuary of Zhu river, Hg and cadmium Cd concentration in Jiaozhou Bay exceeds the envirionmental allowable content and could do serious harm to the aquatic animals. This paper studied effects of heavy metal ions (Cu²⁺, Hg²⁺, Cd²⁺) on Metallothionein (MT) concentration of the gills and hepatopancres, antioxide enzymes activities of three tissues including superoxide dismuse (SOD), catalase (CAT) and glutathione peroxidase (GPx), DNA strand break in three tissues and microstructure of gill filaments and hepatopancrea of Charybdis japonica, meanwhile discussed the toxic and detoxicfied courses of heavy metal ions in Charybdis japonica, in order to provide scientific data for toxicology research on marine creascureas and monitoring the heavy metal contamination in coastal sediment.Effects of three kinds of heavy metal ions on MT concentration in the gills and hepatopancreas of Charybdis japonica during 15 days were studied using using mercury saturation assay method and AAS. The results indicated: The MT concentration in the gills and hepatiopancreas increased significantly in 3 day, then changed significantly with the increasing of metal ions concentrations and prolongation of exposure time(P<0.05)and represented a dose and time relationship during a certain experimental period, whereas that in the control groups had no marked changes (P>0.05) . In addition, the inductive degree of three heavy metal ions in the same concentrions (0.5mg/L) and time on metallothionein concentration in the gills and hepatopancreas were Cd²⁺>Cu²⁺>Hg²⁺ and Hg²⁺>Cd²⁺>Cu²⁺, respectively. And the MT concentration in hepatopancreas was much higher than that in the gills.Effects of three kinds of heavy metal ions on SOD, CAT and GPx activities in the gills and hepatopancreas of Charybdis japonica during 30 days were studied by...