Study On The Biosynthesis Of Bacterial Cellulose By Acetobacter Pasteurianus

Bacterial cellulose synthesized by micro-organisms is a pure form of ultra micro-cellulose cluster that possesses high crystallinity, coincidence, hydrophilicity and strong tensile strength compared with natural plant cellulose. Moreover, good biocompatibility of bacterial cellulose facilitates direct degradation in the nature. Due to its unique properties, it becomes one of the hottest fields of biomaterial research utilizing microbial fermentation to produce bacterial cellulose. And has broad prospects of applications including food, pharmaceutical, paper making and biomedical engineering. In this study, we have established a system for production of bacterial cellulose, and found out the best culture conditions for bacterial cellulose synthesis using Acetobacter pasteurianusoStudy on the influence on bacterial cellulose synthesis by different carbon source medium indicated glycerol was used most efficiently by A. pasteurianus while the usage of ethanol, Acetic and glucose decreased gradually. The trend of pH value of the liquid culture is decreased first and then increased. The optimal growing condition of glycerol as the solo carbon source was 7% inoculum size, 3% glycerol content, and cultivated at 31℃. The growth curve of A.pasteurianus suggested the maximal bacterial amount was achieved on the 8th day of cultivation while the maximal bacterial cellulose production was achieved on the 10th day.