Diversity And Seasonal Dynamics Analysis Of Methanotrophs Population In Activated Sludges

Methanotrophs are widespread in natural habitats, and have attracted the interest of microbiologists due to their potential for use in bioremediation and their importance in the global methane cycle. Two categories of methanotrophs, type I and type II, have been identified using a polyphasic approach. Many previous on bioremediation and degradation of organic pollutants by methanotrophs have focused on well-described pure cultures, However, traditional culture-based can be regarded as artificial microbial communities and exclusive focus on pure cultures may prohibit exploitation of a large fraction of the methane driven degradation potential in natural environments. The purpose of this study is to investigate the diversity of methanotrophs population based on 16S rDNA in activated sludge. DNA was extracted for phylogenetic analysis from activated sludge. The analysis of bacterioplankton community and methanotrophs population by DGGE and RFLP revealed the ecology of activated sludge. It will be the principal foundation of organic pollutants degradation in sewage.In order to extract DNA from activated sludge under simple and easy experimental terms for follow-up PCR analysis, three methods (TENP, high temperature method and optimized PEG) were used and compared, (?)esults showed that the optimized PEG method was the most efficient one.DGGE is applied to compare the microbial community of two activated sludge on different month. The fingerprinting shows that there is not great difference among these samples. The fingerprinting showed a distinct seasonal pattern. The summer community had the highest propotion of bacteria, and a little bacteria lives in winter. Some bacteria had higher proportion in winter, and they were not in summer. Some bacteria approacher gave consistent accounts of seasonal chages in microbial diversity. The major microbial community of two activated sludge belongs to the different gene cluster.We characterized the type I and type II methanotrophs community and its seasonal dynamics in two activated sludge by DGGE analysis of short 16S rDNA PCR productsobtained with primers specific for the domain methanotrophs. The type I...