Research On The Algae-lysing Bacteria H And B Inhibiting The Growth Of Phaeocytstis Globosa And Prorocentrum Donghaiense

In recent years, the apparent increase of red tide has been accompanied with the enlargement of red tide area and more harmful effects on ecological environment and human health. Red tide is a serious global marine disaster. It has been an urgent question how to manage and abate or even prevent red tide efficiently. Presently people mainly use chemical method to prevent red tide, though this method could control red tide rapidly and efficiently, but it brings new contamination to marine environment. In respect that marine bacteria play a great role in the occurrence and declination of red tide based on their population diversity, bio-physiological and biochemical character diversity, ecological function diversity and heredity diversity. Thus, bacteria-algae relationship has been a hotspot in red tide research.Several algae-lysing bacterium were isolated from seawater of Zhu Hai where red tide often breaks out, among which two kinds were labeled as H and B, are algae-lysing bacterium. Their physiological and biochemical properties, algae-lysing specificity and lytic phenomena were investigated. The results showed that two bacteria present a broad algae-lysing range, i.e, they lysed Phaeocytstis globosa and Prorocentrum donghaiense respectively. Colonies of H on solid media (ZoBell 2216E) are yellowish, slightly convex (elevation), entire (margin) and round (configuration); Colonies of B on solid media (ZoBell 2216E) are milky, slightly convex (elevation), entire (margin) and round (configuration). Under the 2216E culture, their growth curves were determined as follows: the lag phase of H and B last about 1h and 3.5h respectively; the stationary phase started as about 25h and 30h respectively. Both of them are gram-positive bacteria. The characters of them for FCM were studied, too.The results showed that different strains of marine bacteria and different concentration of same bacteria strain had different effects on the growth of algae.In this thesis, the effects of H and B on growth of algae under different salinities were studied. It was found that the optimal salinities for H was 20‰. and for B it was 35‰.The features which normal algae cell and damaged algae cell by H and B were...