Atomic Force Microscopay-based Immune Molecules Recognition

The immune recognition of biomolecules should facilitate the disease diagnosis and treatment because many diseases were highly correlated with biomolecular structures and functions. Here, atomic force microscopy (AFM), electrochemistry analyses and TEM were employed to detect and characterize the nanostructures and electrochemistry of biosensing surface.Self-assemble technology was used to design CD29 immunosensor, the immunosensor was detected by modified tips with CD29. Force-distance technology was used to analyze molecular recognision and function dots. The function dots of CD29 immunosensor indicated that 62.5% of electrode surface have obvious function, the others lost function. We can find the antibodies self-assembly to be mass structure by the surface scanning of AFM. The protein lost former structure and it might be the reason of why protein lost function.The method of "nano architecture" (NA) was used to design colloid gold modified electrode. AFM was used to scan the shape of surface molecules and to measure the force of molecular recognition. Electrochemistry was used to testify the electrical property of NA. The surface analysis checked by AFM indicated that the antibodies were distributed well on the surface of electrode. The forece-distance measurement indicated that about 4/5 of electrode surface area were active sites. The association of voltage and current were measured by electrochemistry analysis. From those data, we got the line equation of current variation by concentration, coefficient of association, relative standard deviation, least detectability and so on. All of the experiments indicated that the immunosensor determined by the method of NA were better than traditional simple self-assemble sensor. It also indicated that NA was a useful and creative conception to design immunosensor.We designed a new multipores Si nano-material. The size of nanoparticles is about 15-35nm, which can carry plasmid DNA and protect it from digested by DNA enzyme. The nanoparticles were modified by Tf surface for the -OH on the surface. The plasmid was expressed in HepG2 cells. After 48h, the green fluorescence proteins were expressed in cells which were measured by fluorescent microscope. The fluorescence intensity of modified nanoparticles were higher than unmodified. At last. AFM was used to checkout if the nanoparticles possessed biocompatibility. After...