The Extraction And Separation Of Flavone Components From Jinhuacha Leaves

Jinhuacha [Camellia chrysantha(Hu) Tuyama],is a Sect.ChrysanthaChang, Theaceae,Camellia plant,It is the First - grade State protection and plant endemic species of Guangxi.It has clearing away heat and toxic material,inducing diuresis to reduce edema and prevention of tumor.Now it is used widely in manufacturing medicine, beverage and flavoring expect as a view and admire plant. Predecessors mainly are focused on breeding and Jinhuacha leaves are researched very few. This essay studies Jinhuacha leaves from the flowing four aspects.1. Studied the tea value of Jinhuahca leaves. Jinhuacha leaves contains amino acid,tea polyphenons,flavonoids and such like by qualitative analysis. Amino acid content is determined by ninhydrine colorimetry. Tea polyphenons and ferrous ion can form purple complex, its content is determined by spectrophotometer in 570nm and 540nm. The result shows amino acid content is 5.80%,tea polyphenons content is 9.96%.2. Determined the flavone content of Jinhuacha leaves.The coarse flavone is extracted from jinhuacha leaves by boiling water, collected by resin adsorption, elutriated by 75% ethanol.The refined flavone is obtained by absolute alcohol purification. The content of total flavonoids is measured by external standard method. The result shows that the content of coarse flavone is 2.33% and the content of refined flavone is 4.62%.3. Quercetin and kaempferol are found from jinhuacha leaves by their Retain-time in RP-HPLC. Their monomers are gained by silica gel column chromatography. Three flavonol glycosides and a terpenoid are isolated from Jinhuacha leaves with gradient elution, many times silica gel column chromatography and recrystallization. The structures of the terpenoid and two flavonol glycosides are verified by different kinds of spectrum and chemistry methods. They respectively are kaempferol-3,7-O-α...