| The direct detection method of samples using the spectorphotometry catalyzed by enzyme has been developed. It is applied to analyze the substances in compound water, biscuit and chocolate sugar. The specifically substances catalyzed by Horseradish Peroxidase have been put in the spectrometer and we determine their amount according to the change of the absorption in the specifically wavelength. The method has simple equipment, easy operation and good selectivity. This work includes two parts:1. A method for the determination of Propyl gallate in food catalyzed by Horsera- dish Peroxidase was established. The various analytical conditions are studied. It is based on the decline of product's absorption in 271 nm. The detection ranges were 5~30μg/mL, and the detection limit was 1.25μg/mL. The effects of several important factors such as the acidity of running buffer, the using of reagent and the temperature were investigated. the effect of the coexisted ions was also discussed. This developed method was simple and fast.2. A kinetic spctrophotometric method for the determination of Hg2+ was propos- ed based on the inhibition of Hg2+ for the catalytic reaction of H2O2 -4- aminoantipy- rine-2,4-dichlorophenol systems by horseradish peroxidase. The detection ranges were 1.0~5.0μg/mL, and the detection limit was 5.78×10-7 g/mL. The conditions for the determination of Hg2+was optimized, and the effect of the coexisted ions was dis- cussed. The developed method was simple and fast.
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