Study On The Dissolution Of Phenformin Hydrochloride Tablets

OBJECTIVE: This paper checks the dissolutions of phenformin hydrochloride formations, set up the method for determining the concentration of phenformin hydrochloride in serum .METHODS: The effect of different media on the absorption of phenformin hydrochloride was checked. By adding the same amount of NaOH the influence of HCl can be eliminate. The dissolutions of phenformin hydrochloride tablets formations were determined in H2O and in pH7.0 phosphate buffer solution respectively. The concentration of phenformin hydrochloride in serum was determined using acetonitrile- 0.01mol/L(pH7.0) phosphate buffer solution(40:60) as mobile phase, uBondapak C18(10μm, 250×4.6mm ) as analytical column, 233nm as detective wave. The method was applied to the study of the serum of phenformin hydrochloride.RESULTS and CONCLUSIONS:1 The contents of phenformin hydrochloride formations were 99.52% ; the accumulating dissolution at 45 minetes was95.80%2. The linear range of the method fordetermining the concentration of phenformin hydrochloride in serum was0.079-3.97μg/ml, the recovery was 92.4-95.5%, the RSD within days was92.4-95.5%, between days was 3.81-6.58% respectively.