The Study Of Production Of Beta-Carotene By Blakeslea Trispora

The fermentative conditions ofβ-carotene by Blakeslea trispora have been investigated. These conditions include fermentation medium, the optimization of some fermentation factor. The extracting methods and the TLC of carotenoids were also researched.Firstly, the effects of composition of fermentation medium on the yield ofβ-carotene were studied. the results showed that the best fermentation medium was corn starch 3%,soybean power 2%,cottonseed oil 3%,KH₂PO₄ 0.2%,MgSO₄·7H₂O 0.2%,vitamin B1 0.002%,pH value 6.0. Secondly, through compared some factors, such as different proportion of plus and minus strains, pH value, nonionic surfactants, respective best values have been obtained. The best proportion of plus and minus strains is 1.3:0.7, pH value of fermentation medium (sterilized) is 7.0, fermentation accelerant which acts as surfactants is Triton x-100. Farther on, the fermentative conditions were optimized through orthogonal experiment, the optimization showed that proportion of plus and minus strains is 1.3:0.7,pH value is 7.0, content of Triton x-100 is 0.08%. And the yield ofβ-carotene reached 0.73g/L, which was up to 3.3 times through the fermentation.In the extracting study, it has showed hydrochloric acid-heat treatment is a simple, convenient and effective extracting methods is which was used to destroy the cell wall, and the extracting organic solvent is petroleum ether whose boiling range is 60⁹0℃.In the TLC experiments, extracting contents in the petroleum ether were spotted in the silicagel plate, and the mixed liquor of acetone and petroleum ether (5:95) is developping agent, which can distinguishβ-carotene from other carotenoids. It is a...