Bioaugmentation For Simultaneously Desulfate And Denitrate Process And Analysis On Microbe Community Development

Simutaneous desulfurization and denitrfication process is one of hotspots in wastewater treatment research. Its goal is that realizes simultaneous removal of sulfide and nitrate while the elemental sulfur and the nitrogen gas are produced as metabolic products in the process of wastewater treatment. Combining simutaneous desulfurization and denitrfication process and the bioaugmentation technique with sulfide wastewater process could high effectively remove effluent concentration of sulfide in the process of sulfate wastewater process. On the basis of this research idea, the paper reported research works involving in the following several aspects.A strain of facultative anaerobic bacterium, 3-1F, was isolated from soil using the method of double-layers plate and the Hungate anaerobic operation technique, for which, sulfide is adopted for its electron donor and nitrate for electron acceptor. Identification based on 16S rDNA sequence and physiological biochemical analysis indicated that the isolate has a homologous similarity of 99% with Rhodococcus erythropolis. The characterization of this strain showed that it could reach the logarithmic phase after inoculation for 24 h, and reach a peak after inoculation for 60 h. Under this conditions, the thiosulfate and nitrate removal rate over 94% and 75%, respectively. And the end products of this strain in mainly consist of elemental sulfur and nitrogen gas, as well as small amount of sulfate. Till now, no previous reports metioning the strain of Rhodococcus erythropolis has the function of simoutanous desulfurization and denitrification. The achievement of strain 3-1F provides a useful microbiology resource for the efficiency improvement of simutaneous desulfurization and denitrfication process throught the way of bioaugmentation.In the process of bioaugmentation experiment, the strain 3-1F was put into the continuous flow bio-reactor. The result of bioaugmentation experiment showed that the operation had not obvious effect on pH, alkalinity, COD and nitrate removal rate. The bioaugmentation operation had produced a short term effect on sulfate removal rate, concentration of effluence sulfide decreased from 130mg/L to 30mg/L in the bioaugmentation experiment within 2d. Meanwhile,...