| Puerarin is an active constituent extracted from the traditional Chinese herb Radix Pueraria lobata Ohwi and P.thomsonii Benth, which has been widely used to treatcardiovascular and cerebrovascular diseases. Due to its poor water solubility and poor absorption after oral, its clinical therapeutic effect has been limited. Microemulsion drug delivery system(MEDDS) is a kind of new drug delivery system, which has been received great attention in recent years. It has been used as a vehicle to increase the absorption of insoluble drugs. Therefore, microemulsion (ME) was selected as the carrier to increase dissolution of puerarin, which would increase absorption and bioavailability of the drug. The study hasn't been reported .The saturated solubility of puerarin in different oils and surfactants, and the interaction between oils, surfactants and cosurfactants were investigated. The optimized formulation of the blank microemulsion selected by pseudo tertiary phase diagrams consisted of Tween80-glycerin-ethyl oleate-water at weight ratio of 20:20:1.6:58.4. Puerain microemulsion with drug concentration of 20mg·ml-1 was prepared based on the blank microemulsion.Though comparing the solubility of ME, surfactants micelle solution and water solution, we validated that the ME could improve the solubility of drug evidently. The ME presented as small spherical drops under electron microscopy, with the average diameter 23.4nm. Its basic character parameter such as viscosity, refraction, electric conductivity, Zeta potential were (13.4±0.12)mm2·s-1 (1.3912±0.00005) (112±1.52)s·cm-1 ,-0.53mV, respectively. The test of influencing factors and primary stability test showed that the puerain in ME had a better stability, and it could keep stable at room temperature.
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