The Preparation And Property Research Of Pretein Combined Biological Active Hydrogels

Most of pretein,polysaccarides and lipid materials come of natral products,there are lots of sources of them,and they are nongerm,nonpoison,of excellent biocompatible and biodegradable.So they have become basic material of extensive research and being used of drugs and packing fields.Materials of only pretein,because of their inherent sensitivity to temperature and pH,and their poor machining property,they are of limited application; However,other composite polymer materials are of poor water-solubility,few activefunction-groups,complex reaction and high cost of operation.At present,there are very few resarchs of pretein-polysaccaride polymer materials as carrier . Our research is to develop a new pretein-polysaccaride polymer hydrogel delivery materials,taking use of pretein-polysaccarides combined with biodegradable polymer,we can improve materials'hydrophile,and extend molecule dimension to realize drug-delivery slowly,and we can also combine all kinds of materials of biological active easily with abundant activefunction-groups of natural materials,meanwhile improving pretein-polysaccaride polymers,we can extend their application in the machiningable-materials field..The outline of the research as follow:1. Study on the synthesis of N-glycinylmaleimid. Among lots of modificative reagents,N-glycinylmaleimid can be used as modificative reagent,and also can be used as intermediate of synthesizing complex modificative reagent,it is a useful modificative reagent.In order to synthesize the modificative reagent,four methods are tried:1) 6-Maleimido caproic acid was synthesized by the reaction of 6-aminocaproic acid andmaleic anhydride at room temperature in 5. 0 h by using acetic anhydride and sodium acetate as a catalyst.2) 6-Maleimido caproic acid was synthesized by the reaction of 6-aminocaproic acid andmaleic anhydride using triethylamine to dehydrate.3) 6-Maleimido caproic acid was synthesized by the reaction of 6-aminocaproic acid andmaleic anhydride by the method of two steps . 4)N-glycinylmaleimide(GMI) was synthesized by usingmaleic anhydride and glycine by the method of two steps.2.The preparation of CTS-SH.Bringing–SH to chitosan to research the modification of chitosan.We prepared a new evolution of CTS by modifying CTS with thioglycolic acid,acetic anhydride and oil of vitriol,and certified its structure with IR and H-NMR.At the same time,we improved the experimental conditions in plenty of experimentations.3. The research pretein- chitosan hydrogels.To synthesize hydrogels with BSA and PHCSSA,first need to activate PHCSSA's carboxyl quantificationally , then synthesize different kinds of hydrogels with different drippy order:pectinate hydrogels were prepared by dripting active PHCSSA solution to BSA solution;while netty hydrogels were prepared by dripting BSA solution to active PHCSSA solution.And netty hydrogels of different BSA's content were prepared according to the method.In addition,crosslinked hydrogels of only BSA itself were prepared with glutaradeliyde as crosslinker.Finally,contrast the structure and property of all the hydrogel above,the result is:pectinate hydrogels are of better water-swelling property and incompact spatial structure . Hydrogel systems can absorb druggery molecules effectively,their release rates and release quantities of under acidic condition are lower obviously than under litmusless or alkalescent condition,they put up pH sensitivity,and at last,the release effect of pectinate hydrogels is the best.